Journal: Quantitative Biology

Article Title: Metabolic‐immune interactions in gastric cancer T cells: A single‐cell atlas for prognostic biomarker identification

doi: 10.1002/qub2.70027

Figure Lengend Snippet: RT‐qPCR showing the mRNA expression of (A) ZNF207 , (B) ZNRF1 , (C) RGS1 , (D) ARPP19 , (E) CXCR4 , and (F) CTLA4 in paracancerous (principal component, PC) tissues ( n = 10) and gastric cancer (GC) samples ( n = 10). * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Immunohistochemical staining was then carried out using primary antibodies targeting RGS1 (Sigma, HPA028453), CXCR4 (Cell Signaling Technology, #97680), CTLA4 (Cell Signaling Technology, #53560), ARPP19 (Proteintech, 11678‐1‐AP), ZNRF1 (LSBio, LS‐C809970), and ZNF207 (Invitrogen, PA5‐53535).

Techniques: Quantitative RT-PCR, Expressing

Journal: Quantitative Biology

Article Title: Metabolic‐immune interactions in gastric cancer T cells: A single‐cell atlas for prognostic biomarker identification

doi: 10.1002/qub2.70027

Figure Lengend Snippet: Immunohistochemical staining of target genes (A) ZNF207 , (B) ARPP19 , (C) ZNRF1 , (D) CXCR4 , (E) RGS1 , and (F) CTLA4 expression profiles in gastric cancer specimens and their corresponding adjacent normal gastric tissues. Brown chromogenic staining reveals positive protein expression. Blue counterstaining visualizes cell nuclei. Scale bar: 100 μm.

Article Snippet: Immunohistochemical staining was then carried out using primary antibodies targeting RGS1 (Sigma, HPA028453), CXCR4 (Cell Signaling Technology, #97680), CTLA4 (Cell Signaling Technology, #53560), ARPP19 (Proteintech, 11678‐1‐AP), ZNRF1 (LSBio, LS‐C809970), and ZNF207 (Invitrogen, PA5‐53535).

Techniques: Immunohistochemical staining, Staining, Expressing

Journal: Cancer Science

Article Title: MASTL Promotes Hepatocellular Carcinoma Progression and Paclitaxel Resistance Through Mitotic Catastrophe

doi: 10.1111/cas.70287

Figure Lengend Snippet: MASTL regulates mitotic catastrophe via the PP2A‐B55α pathway. (A) ENSA and phospho‐ENSA (Ser67) expression after MASTL modulation. (B) PP2A‐B55α protein levels following MASTL perturbation. (C) PP2A phosphatase activity in MASTL‐modified cells. (D) Validation of PP2A‐B55α knockdown efficiency by WB. (E–G) Rescue of MASTL depletion phenotypes by PP2A‐B55α co‐knockdown: (E) Cell viability (CCK‐8), (F) Mitotic catastrophe (α‐tubulin/DAPI), and (G) Paclitaxel response.

Article Snippet: After blocking with 5% skimmed milk in TBST for 2 h at room temperature, the membranes were incubated overnight at 4°C with the following primary antibodies: anti‐MASTL (Cat# ab86387, Abcam), anti‐γ‐H2AX (Cat# 9718T, Cell Signaling Technology), anti‐Caspase‐2 (Cat# ET1602‐30, Huabio), anti‐Cleaved‐Caspase‐2 (Cat# R381055, Zenbio), anti‐ENSA (Cat# ab180513, Abcam), anti‐Phospho‐ENSA (Ser67) (Cat# 5240S, Cell Signaling Technology), anti‐PP2A‐B55α (Cat# A24261 , ABclone), and anti‐E2F1 (Cat# 66515‐1‐Ig, Proteintech).

Techniques: Expressing, Activity Assay, Modification, Biomarker Discovery, Knockdown, CCK-8 Assay

Journal: The Journal of Biological Chemistry

Article Title: Characterization of Mast2 kinase defines structural features, regulation, and substrates

doi: 10.1016/j.jbc.2025.110922

Figure Lengend Snippet: Mast2 (294–1197) robustly phosphorylates S67-ENSA. A, the site-specific information of selected significantly upregulated peptides from B ( green box ). B, nonradiolabeled substrate assay of ENSA (MCE) phosphorylated by Mast2 (294–1197). Total ENSA and FLAG immunoblot are shown with a representative pS67-ENSA blot ( right ), and phosphorylation was quantified by percent maximal AU with each aligned data point shown over a nonlinear curve determination ( left ). K m was calculated using GraphPad Prism. MAST, microtubule-associated serine/threonine; ENSA, endosulfine-α.

Article Snippet: Primary antibodies for total AKT (2920S), phospho-specific AKT (pT308, 9275S and pS473, 4060L), phospho-specific S6K (pT389, 9205S), phospho-specific ENSA (pS67, 5240S), and total ENSA (8770S) were from Cell Signaling.

Techniques: Western Blot, Phospho-proteomics